Maddie Scrupps (2018-Present)

Maddie is a sophomore researcher at the ASR Laboratory engineering a novel casein protein detection strip. About 2.5 percent of children under three years old are allergic to milk. Nearly all infants who develop an allergy to milk do so in their first year of life. In a recent study of 52,000 US households, found that nearly 2% of children have an allergy to milk. Casein is the main protein in milk; it makes up 82% of the proteins. In the United States, 5.6 million children have dairy allergies, and 20% of those children will not outgrow them. Industries such as Neogen have been developing commercial brand casein test strips, but the wait time for users can be between 5-10 minutes per sample. This research is developing a casein test strip that could identify the milk protein casein using an ELISA protocol. The time of analyzing would decrease due to ELISA using a specific detection method that uses antibodies, and visual detection. The commercial brand (Neogen-the controlled group) had an average analyzing time of 4 min, 47 s. The ELISA protocol needs different reagents to analyze the proteins. Instead of using a basic milk wash, a novel wash reagent was used. In the first phase, the experimental strips did not out-perform the control strips for total time, but did end up costing less per unit. The second phase is currently being researched and will hopefully have a statistically lower average analyzing time. During the 2017-2018 year, Maddie was selected by The Rural Alliance for their STEM Gems program, advocating STEM success for rural students interested in science or engineering careers. Maddie's outstanding research earned her 1st Place Awards at both the Regional and State Science Fairs.

Research:
Novel Casein Detection Indicator
- ASR Laboratory, Odessa High School, Odessa, WA 2019

Recognition:

2019
- 1st Place Senior Division Award in the Category of Biomedical Sciences, Bremerton, WA
- 1st Place Senior Division Award in the Category of Biomedical Sciences, Spokane, WA
- Outstanding OHS Advanced STEM Researcher Award, Odessa High School, Odessa, WA
- STEM Gems recipient from The Rural Alliance, Spokane, WA

2018
- 1st Place OHS Peer Award for Science Research in Biology, Odessa, WA
- STEM Gems recipient from The Rural Alliance, Spokane, WA

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